STUDIES IN OXIDATIVE PHOSPHORYLATION WITH RADIOACTIVE PHOSPHATE

Reduction studies with naturally occurring quinones and their phosphate derivatives with reference to oxidative phosphorylation.

Plastoquinol dibenzyl-32P-phosphate was prepared by reduction of plastoquinone with dibenzyl-32P-phosphonate. Catalytic hydrogenation of the product resulted in the reduction of the polyisoprenoid side chain as well as debenzylation. Substantial reduction was also observed with the use of nuclear magnetic resonance for a number of naturally occurring quinones with conditions recommended for qui...

Studies of factors involved in oxidative phosphorylation.

The oxidation of substrates of the citric acid cycle in mitochondria is coupled with the phosphorylation of ADP to ATP.I Considerable progress has been made in our understanding of the three electron transport reactions between DPNH and oxygen (DPNH -.cytochrome b -. cytochrome c -° 02), which are associated with the phosphorylation mechanism.2 In several laboratories3-7 actively phosphorylatin...

Further studies on oxidative photosynthetic phosphorylation.

Studies on the Mechanism of Oxidative Phosphorylation

Kinetic and nucleotide binding studies have shown that submitochondrial particles from bovine heart possess three exchangeable binding sites for ADP or GDP. In order of decreasing affinity at neutral pH, these sites will be referred to as sites I, II, and III, and their respective dissociation constants as KI, KIr, and KIII. In oxidative phosphorylation experiments in the presence of saturating...

Phosphate Dependence and Atractyloside Inhibition of Mitochondrial Oxidative Phosphorylation

ATP production and oxygen consumption by rat liver mitochondria were measured as a function of atractyloside and phosphate (Pi) concentration. The “on” kinetics of atractyloside inhibition of ATP production are very rapid, and the onset of inhibitory effect is complete within 1 s even at concentrations which produce partial inhibition. A ligand conservation plot relating atractyloside concentra...